Turtle Technology

21/07/2025

Unifying Nucleic Acid and Protein, Thermal and Isothermal: The Launch of the Digital Omni Molecular Detection System

At the end of June 2025, Turtle Technology officially launched the Digital Omni Full-Modality Molecular System in Shanghai. This groundbreaking platform is the first in the industry to integrate three major functions into a single system: digital PCR detection (thermocycling-based nucleic acid testing), digital isothermal detection (isothermal nucleic acid testing), and digital single-molecule immunoassay (protein detection based on immuno-PCR principles). This signifies the beginning of a new era of unified molecular diagnostics.

In cutting-edge molecular detection applications, researchers often require multiple different systems to test both nucleic acids and proteins. This leads to high equipment investment and operational challenges in terms of workflow, data integration, and quality control. As the industry's first truly unified detection platform, the Digital Omni system solves several key technical challenges and takes molecular diagnostics to a new level.

Core Innovation Through Unique Construct of Technological Integration:

The Digital Omni System is built on Turtle Technology’s highly open and compatible core platform, which includes proprietary innovations such as "solid-state oil-water separation" droplet generation, sandwich-structured microfluidic chips, and all-in-one automated instrumentation.

Digital PCR Mode: Enables high-precision absolute quantification of nucleic acids. Combined with exponential ultra-multiplexing technology, it allows the detection of over 15–30 targets per tube.

Digital Isothermal Mode: Compatible with multiple isothermal amplification methods such as LAMP, RPA, ERA, and SAT, completing quantification in as little as 25 minutes.

Breakthrough in Digital Single-Molecule Immunoassay:

The most significant innovation lies in its integration of digital single-molecule protein detection, unifying what were traditionally separate systems for nucleic acid and protein analysis. This development paves a new path for molecular diagnostics.

While traditional protein assays such as ELISA are limited in sensitivity, Digital Omni adopts a next-generation immuno-PCR-based method that suppresses background noise and significantly improves the signal-to-noise ratio. As a result, it achieves detection sensitivity at the fg/mL level, improving sensitivity by 100 to 1000 times compared to conventional ELISA — all while maintaining a comparable cost. This enables true single-molecule protein detection at low cost, unlocking new potential for ultra-sensitive biomarker testing.

Moreover, the platform is highly open and compatible with a range of protein detection techniques:

- New binding-induced reaction methods such as BIOSEER (Binding-Induced Oligonucleotide Successive Exchange and Extension Reactions)
- Traditional PLA/PEA (Proximity Ligation/Extension Assays)
- Immuno-PCR based on classic sandwich ELISA
- Super-sensitive quantification of pathogenic protein aggregates using Digital Seed Amplification Assay (dSAA)

A New Era of Unified Molecular Detection

The launch of the Digital Omni System demonstrates the feasibility of deeply integrating various detection technologies within a single platform. Its fusion of nucleic acid and protein detection sets a pioneering example for the industry and reflects the strong innovation capacity of Chinese life science companies.

With its all-in-one design, Digital Omni significantly reduces equipment and maintenance costs, eliminating the need for multiple devices and specialized personnel. It effectively lowers the barrier to entry for advanced diagnostics, making single-molecule testing more accessible and applicable across a wide range of fields.

Learn more about Digital Omni:
👉 https://www.turtle-tech.com.cn/?Digital-Omni-cn/Digital-Omni-cn.html

Check us out:
Web: www.turtle-tech.com.cn
Facebook: https://www.facebook.com/profile.php?id=61552564289611
YouTube: https://www.youtube.com/channel/UC7SiBbiwL38iYw0lOGrwvKA
Contact us:
Tel: +86-21-36583658
Mobile: +86-18001988905 (WhatsApp & WeChat)
Email: [email protected]

21/07/2025

Innovative Breakthrough: Turtle Technology Leads the Integration of AI Neural Networks with Digital PCR

Recently, a joint research team from Fudan University and Shanghai Turtle Technology Co., Ltd. published a groundbreaking paper in the internationally renowned journal Sensors & Actuators: B. Chemical, titled "R³Net: A three-phase neural network for noise elimination and accurate quantification in chip-based digital PCR" (https://www.sciencedirect.com/science/article/abs/pii/S0925400525008998). This achievement marks a significant milestone in Turtle Technology’s development in the BioAI (biological artificial intelligence) field and showcases its commitment to its mission of “Leading Life Science and Molecular Diagnostics into Digital Era.”

Traditional chip-based digital PCR (cdPCR) systems face inherent challenges such as fluorescence noise, dark field interference, and light leakage during experimental operations and image acquisition. These issues can significantly compromise the precision and reliability of the results. Existing image processing methods often struggle to cope with such complex noise, which has become a bottleneck for further advancement of digital PCR technology.

Turtle Technology’s R³Net (Recognition-Restoration-Reading Net) revolutionizes this space with a novel three-phase neural network architecture. It employs a sequential process consisting of noise recognition, image restoration, and chip reading. The first step uses a U-Net-based model to accurately identify noise regions and generate noise masks. Next, a novel spiking residual network (S-SRNet) performs image restoration. Finally, a lightweight YOLO-mini network executes high-precision quantification. The unique temporal input mechanism enables the model to effectively distinguish between foreground noise and background regions, enhancing noise processing precision. Additionally, the integration of a spiking neural network (SNN) not only boosts computational efficiency but also strengthens temporal sensitivity, offering a new solution for image processing in noisy environments.

The experimental performance is outstanding. The research team conducted extensive tests using DNA samples from lung cancer, COVID-19, and influenza viruses. R³Net significantly outperformed conventional methods across key indicators: it achieved an accuracy of 88.47% in noisy image scenarios, with image clarity and similarity metrics reaching 41.38 and 99.72%, respectively. In terms of speed, the lightweight algorithm maintained a high accuracy of 98.27% while reducing system resource consumption by over 98%. It processes a single image in just 1.3 seconds—meeting the rapid demands of clinical diagnostics.

This technological breakthrough not only advances the academic frontier but also holds immense commercial and market value. As a leading provider of digital PCR and digital molecular diagnostics solutions in China, Turtle Technology offers a full range of products from the BioDigital Mini to the fully automated SCI Digital Pro. The successful integration of AI technology injects powerful new capabilities into this product ecosystem. For end-users, the R³Net-enhanced platforms promise more stable and reliable results even in challenging lab environments.

The successful application of R³Net signals the beginning of an AI-driven era in digital PCR and marks the first step in Turtle Technology’s BioAI roadmap. As a co-founder of the global life science AI community BioBuddy, Turtle Technology is actively building a diverse product portfolio including the BioSalesAI digital assistant solution and a dedicated training center for life science AI. Researchers and industry partners interested in collaborating are welcome to connect.

Check us out:
Web: www.turtle-tech.com.cn
Facebook: https://www.facebook.com/profile.php?id=61552564289611
YouTube: https://www.youtube.com/channel/UC7SiBbiwL38iYw0lOGrwvKA
Contact us:
Tel: +86-21-36583658
Mobile: +86-18001988905 (WhatsApp & WeChat)
Email: [email protected]

Turtle Technology is committed to leading the life sciences and molecular diagnostics into the digital era. Having been approved as the National Demonstration Center for Genetic Testing Technology and obtaining the first Digital PCR Metrological Evaluation Certificate, we have introduced a variety o...

21/07/2025

European Partner Visits Turtle Technology to Strengthen Strategic Collaboration on Ultra-Multiplex Digital PCR

Shanghai, China – June 5, 2025 – Turtle Technology welcomed an important delegation from one of its most strategic European partners to its headquarters. Since establishing their partnership in 2023, both parties have maintained close communication and cooperation, jointly enhancing Turtle Technology’s brand visibility and market pe*******on in the European region.

During the visit, the two sides engaged in in-depth discussions on the trends and competitive landscape of digital PCR technologies, the technical advantages of Turtle Technology’s core product lines, and future regulatory and collaborative strategies. In particular, the visiting partner expressed strong recognition for Turtle Technology’s next-generation digital PCR platform, SCI Digital Pro. The platform impressed with its significantly enhanced automation, throughput, AI-assisted data analysis capabilities, and especially its expanded Ultra-Multiplex detection capacity, which enables simultaneous quantification of up to 63 targets — a breakthrough that received high praise from the visiting guests.

In addition, both parties explored future plans related to regulatory pathways, market expansion strategies, joint product development, and opportunities for localized applications. They also reached a preliminary consensus on the next phase of their strategic collaboration.

Check us out:
Web: www.turtle-tech.com.cn
Facebook: https://www.facebook.com/profile.php?id=61552564289611
YouTube: https://www.youtube.com/channel/UC7SiBbiwL38iYw0lOGrwvKA
Contact us:
Tel: +86-21-36583658
Mobile: +86-18001988905 (WhatsApp & WeChat)
Email: [email protected]

21/07/2025

Turtle Technology and GEB Sign Strategic Cooperation Agreement

On April 30, 2025, Turtle Technology and GEB signed a strategic cooperation agreement in Shanghai. This cooperation marks the deep integration of both parties in fields such as low-volume nucleic acid extraction and enrichment technologies, Ultra-Multiplex digital PCR quantitative detection technologies, and the joint establishment of a "Digital PCR+" innovation platform. By joining forces, the two companies will work together to open a new chapter in life sciences and precision medicine.

Both parties will leverage their respective technological and resource advantages, and engage in multi-level and comprehensive collaboration in areas such as technology co-development, integration of products and application solutions, domestic and international business coordination, and joint global expansion. Together, they aim to promote the implementation and transformation of low-volume nucleic acid extraction and enrichment, and ultra-multiplex nucleic acid quantitative detection technologies in application scenarios such as scientific research, food safety, animal and plant inspection and quarantine, public health, and clinical practice, contributing to the development of life sciences and precision medicine in China and globally.

This strategic cooperation is not only an important embodiment of mutual benefit and win-win collaboration, but also a meaningful practice in promoting continuous innovation in high-end domestic life science and medical diagnostic products. In the future, Turtle Technology and Jinxin Biotech will continue to embrace the industry with a spirit of openness, collaboration, and innovation, jointly welcoming new opportunities in the life sciences sector.

Check us out:
Web: www.turtle-tech.com.cn
Facebook: https://www.facebook.com/profile.php?id=61552564289611
YouTube: https://www.youtube.com/channel/UC7SiBbiwL38iYw0lOGrwvKA
Contact us:
Tel: +86-21-36583658
Mobile: +86-18001988905 (WhatsApp & WeChat)
Email: [email protected]

19/02/2025

Breaking Limits: Turtle Technology Launches Customized Development Service for Ultra-Multiplex Digital PCR

Ultra-Multiplex Digital PCR, based on Turtle Technology's proprietary multi-color fluorescence encoding and decoding technology, utilizes a multi-color fluorescence digital PCR system to enable simultaneous detection of 10-30 targets within a single chip. This technology not only facilitates qualitative detection but also enables precise quantitative analysis, offering an innovative and highly practical solution.

Ultra-Multiplex Digital PCR is highly suitable for multiple pathogen detection, such as respiratory, gastrointestinal, bloodstream infections, environmental microbes, vector-borne diseases, animal epidemic diseases, foodborne pathogens, and drug-resistant genes. It is also ideal for multi-gene expression quantification and multi-gene genetic testing for hereditary diseases, such as deafness-related genes.

To better serve our customers and promote the application of Ultra-Multiplex Digital PCR technology, Turtle Technology is offering custom development services for Ultra-Multiplex Digital PCR products.

This service caters to life science researchers, corporate product R&D, customs and disease control, foodborne pathogen detection, and more.

We have already successfully developed 28-plex respiratory pathogen detection systems, 13-plex Vibrio pathogen detection systems, 20+ diarrhea detection systems, and 50+ gene expression quantification systems. With an experienced development team, we can accelerate and enhance your product development process.

Additionally, we are open to collaborating on research project applications, patent filings, and research paper publications. Through our “Super Life Sciences Cooperation Program,” research achievements can be rewarded, and for products with commercialization potential, we also provide outsourced production and product registration services for follow-up results.

Moreover, Turtle Technology, in partnership with BioBuddy, has launched the "Ultra-Multiplex Quantitative PCR Training Academy", aimed at promoting the widespread use and technical exchange of Ultra-Multiplex PCR. Stay tuned for upcoming sessions!

Turtle Technology – your expert in Ultra-Multiplex Digital PCR technology!

For more information about Ultra-Multiplex, please see:
https://www.turtle-tech.com.cn/?technology-en/

Check us out:
Web: www.turtle-tech.com.cn
Facebook: https://www.facebook.com/profile.php?id=61552564289611
YouTube: https://www.youtube.com/channel/UC7SiBbiwL38iYw0lOGrwvKA
Contact us:
Tel: +86-21-36583658
Mobile: +86-18001988905 (WhatsApp & WeChat)
Email: [email protected]

21/01/2025

From False Positives to Accurate Diagnosis: Digital PCR Technology Reshapes the New Paradigm in SMA Genetic Testing

Recently, Professor Xiangdong Kong's team from The First Affiliated Hospital of Zhengzhou University published an important research finding in the prestigious journal Orphanet Journal of Rare Diseases. This study provides a comprehensive gene analysis of 680 suspected SMA (Spinal Muscular Atrophy) families, marking it as the largest SMA genetic diagnostic study globally. The findings provide crucial insights for the diagnosis and treatment of this rare disease.

The research team has also developed a complete genetic diagnostic workflow, which integrates multiple technologies including MLPA, digital PCR, and sequencing. This comprehensive approach allows for a thorough assessment of the genetic status of patients. Notably, this multi-technology strategy significantly enhances the accuracy and comprehensiveness of the diagnosis, particularly in complex cases.

Unique Contributions and Innovative Value of the Study:
1. Largest Cohort Study

- First systematic gene analysis of 680 Chinese SMA families

- Identification of several new pathogenic gene mutations

- Establishment of a complete genetic diagnostic workflow

2. Discovery of Multiple Gene Coexistence Phenomenon

- In addition to the SMN1 gene, pathogenic mutations were found in IGHMBP2, SCO2, and NEB genes

- Expanded the genetic understanding of SMA and provided new perspectives for clinical diagnosis

3. Innovative Application of Digital PCR Technology

- First systematic validation of the accuracy of digital PCR in SMA diagnosis

- Successfully detected and corrected false-positive results from MLPA testing

Additionally, the team previously used single-cell direct amplification digital PCR technology for screening SMA (2+0) carriers in s***m cells, demonstrating the immense application value of digital PCR in SMA detection and genetic screening.

These groundbreaking research results are based on Turtle Technology’s digital PCR platform.

Turtle Technology has successfully developed an SMA (SMN1 & SMN2) detection kit based on digital PCR technology, enabling highly accurate SMA detection and screening to support genetic reproductive health.

For more information about SMA detection kits, please go to:
http://www.turtle-tech.com.cn/newscen1/3629.html
For more information about Turtle Tech's digital PCR panels, please go to:
http://www.turtle-tech.com.cn/test-kits-en/catalog-en.html
Check us out:
Web: www.turtle-tech.com.cn
Facebook: https://www.facebook.com/profile.php?id=61552564289611
YouTube: https://www.youtube.com/channel/UC7SiBbiwL38iYw0lOGrwvKA
Contact us:
Tel: +86-21-36583658
Mobile: +86-18001988905 (WhatsApp & WeChat)
Email: [email protected]

06/12/2024

Turtle Technology Launches Gene Editing Efficiency Detection Kit

Gene editing often requires testing for editing efficiency. However, existing methods, such as T7E1 enzyme cleavage analysis, fluorescent quantitative PCR, and DNA sequencing, are typically cumbersome, time-consuming, and lack precision.

To address these challenges, Turtle Technology introduces the Gene Editing Efficiency Detection Kit (qPCR & dPCR Methods).

This kit utilizes ultra-high specificity Taq DNA polymerase——SNUPP polymerase and the third-generation ARMS technology, the Perfect-ARMS (http://www.turtle-tech.com.cn/SNUPP/SNUPP-en.html). With just one qPCR or dPCR reaction, you can quickly and accurately measure gene editing efficiency.

Highlights of the Gene Editing Efficiency Detection Kit:
- Easy to Operate: One-step procedure for rapid and reliable gene editing efficiency data.
- High Specificity: Precisely distinguishes between wild-type and mutant sequences, suitable for detecting various types of gene edits.
- Broad Applicability: Supports CRISPR/Cas9, TALEN, and ZFN technologies (including indel detection, HDR repair, and base editing) and is compatible with various biological samples.

Key Features of the SNUPP DNA Polymerase:
- Ultra-High Specificity:
Specific primer recognition with the ability to detect SNPs using just one 3' terminal base mismatch. Ideal for third-generation ARMS technology.
- Compatible with Taqman Probes:
Features 5' exonuclease activity, making it suitable for qPCR using Taqman probes.
- Low Non-Specific Amplification:
Minimizes non-specific amplification, reducing qPCR background signals and droplet noise in digital PCR.
Requires lower primer specificity, simplifying primer design.
- High GC Template Compatibility:
Excellent performance in high-GC content systems (up to 85%) and poorly conserved primer regions.
- Wide Range of Applications:
Suitable for high-specificity/multiplex PCR, fluorescent quantitative PCR (dye-based and probe-based), and digital PCR (probe-based).

Comparative Amplification Specificity of Different Taq Enzymes:
(Figure 2) HEX Channel (Indel-Insensitive Amplicon to Indicate Amplification Activity): The amplification activity of all Taq enzyme products is comparable.

(Figure 3) FAM Channel (Indel-Sensitive Amplicon to Indicate Specificity): SNUPP Taq enzyme shows no amplification signals in indel mismatch templates, demonstrating specificity superior to other products by at least 27 cycles (Ct).

For more information about SNUPP polymerase, please go to:
http://www.turtle-tech.com.cn/SNUPP/SNUPP-en.html

Check us out:
Web: www.turtle-tech.com.cn
Facebook: https://www.facebook.com/profile.php?id=61552564289611
YouTube: https://www.youtube.com/channel/UC7SiBbiwL38iYw0lOGrwvKA
Contact us:
Tel: +86-21-36583658
Mobile: +86-18001988905 (WhatsApp & WeChat)
Email: [email protected]