Next Generation Fluorescence Imaging NGFI

15/11/2017

K+ plays an important role in physiology and disease, but the lack of high specificity K+ sensors limits our understanding of its spatiotemporal dynamics. Here the authors develop genetically-encoded FRET-based probes able to quantify K+ concentration in body flui...

14/11/2017

The first genetically encode potassium probes allow visualizing subcellular potassium dynamics in single cells and living animals. Now available at the NGFI store.

K+ plays an important role in physiology and disease, but the lack of high specificity K+ sensors limits our understanding of its spatiotemporal dynamics. Here the authors develop genetically-encoded FRET-based probes able to quantify K+ concentration in body flui...

19/09/2017

The geNOps technology highlights obvious differences in the kinetics, amplitude and substrate-dependence of cellular NO signals-derived from all three nitric oxide synthase isoforms.

The members of the nitric oxide synthase (NOS) family, eNOS, nNOS and iNOS, are well-characterized enzymes. However, due to the lack of suitable direct NO sensors, little is known about the kinetic properties of cellular NO generation by the different nitric oxide synthase isoenzymes. Very recently,...

25/03/2017

Visualized experiments demonstrating the application of the geNOps technology.

https://www.jove.com/video/55486/application-genetically-encoded-fluorescent-nitric-oxide-no-probes?status=a57492k

08/12/2016

Researchers give a link between mitochondrial Calcium uptake and Nitric Oxide formation in Endothelial Cells, using the novel geNOps Technology.

Highlights Mitochondrial Ca2+ uptake regulates diverse endothelial cell functions and has also been related to nitric oxide (NO•) production. However, it i

04/12/2016

Austrian researchers are discovering new details on the vasodilating effects of nitroglycerin using the novel geNOps technology.

Latest News from NGFI and the Scientific Community. Latest News and Publications for Live-Cell Researchers and Cell-biologists. NGFI News...

01/09/2016

Much to our regret we had to hear that Roger Tsien, one of the most outstanding scientist of our time and Nobel Prize winner 2008, died on August 24. Tsien was a brilliant UC San Diego researcher and the father of fura-2 and initiator of FP-containing fluorescence sensors and although being a real genius that brake new grounds not only in fluorescence research but also cell biology and cancer research, he was a modest friendly person that was open to every science discussion. He truly was ahead of all of us and he will be sorely missed as scientist for his revolutionary spirit that inspired several generations of scientist. With his death we lost one of the most shining personality in our science.
He will not be forgotten and we stay thankfully and deeply indebted to this brilliant genius and great man.
The NGFI Team

30/03/2016

http://www.nature.com/nmeth/journal/v13/n4/full/nmeth.3827.html

Researchers have developed a set of fluorescent-protein-based sensors for endogenous nitric oxide.

04/02/2016

http://www.nature.com/ncomms/2016/160204/ncomms10623/full/ncomms10623.html

Nature Communications | Article Open Print Share/bookmark Cite U Like Facebook Twitter Delicious Digg Google+ LinkedIn Reddit StumbleUpon Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics Emrah Eroglu1, Benjamin Gottschalk1, Suphachai Charoensin1, Sandra Blass1, Hel…

07/10/2015

http://www.genengnews.com/gen-news-highlights/nobel-prize-in-chemistry-awarded-to-dna-repair-researchers/81251830/

Three Nobel Prize in Chemistry recipients made fundamental contributions to the study of how cells repair DNA and maintain genomic integrity.