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observed population profiles in most tumors, suggesting the presence of recently activated proliferating and non-senescent intratumoral cells with potential for therapeutic rejuvenation.

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Figure Thumbnail Figure Figure Human Contains Exhausted Show Full Caption View Larger Image Figure Browser Download High Resolution Image Download Next, we studied

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dysfunction in the context of this disease. Humans contain a similarly depleted intratumoral cell population To investigate whether these observations could be extended to humans, we
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maps and, consistent with our clinical consistent with the previous analysis. with our mouse model Consistently, we observed that eventually exhausted graphs are present in most
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isolated intratumoral cells from freshly resected surgical samples for flow cytometry analysis. Of the samples evaluated, 4 had sufficient range for further immunophenotyping. Consistent

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Chihara et al., Schittinger et al., we examined co-inhibitory receptor expression on observed clusters had higher gene expression profiles associated with cellular depletion. Then we

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