14/04/2026
A Drop-by-Drop Self-Assembled All-Natural Hydrogel as a Desensitizer for Rapid and Enduring Management of Dentin Hypersensitivity (5)
Cytoprotective effect of DT occlusion against acid attack. (A) Schematic illustration depicting the modified Transwell dentin disk (modified-TDD) model employed to detect both the cytocompatibility and cytoprotective effect of the ST10 hydrogel to HDPSCs. In the upper chamber, the conventional Transwell chamber membrane was replaced by our dentin disks, and HDPSCs were cultured in the lower chamber to detect whether the citric acid solution from the upper chamber could traverse the modified chamber membrane and affect cell viability in the lower chamber. (B) Photos displaying the upper chambers of the modified-TDD model, where the Transwell chamber membrane was replaced by (B1) a Titanium chip in the blank group, (B2) a dentin slice in the ST10 group and (B3) an EDTA-etched dentin slice in the control group. (C) Cytotoxicity results for HDPSCs cultured in the modified-TDD model using the CCK-8 assay. The OD values were calculated to evaluate the cytocompatibility using one-way ANOVA followed by Tukey’s post-hoc test (n = 3, ns = not significant, *p < 0.05 against the control group) (D-F) Fluorescence microscopy images of live/dead HDPSCs within the modified-TDD model. The corresponding inset photos display the lower chamber of the modified-TDD, with cells and culture medium replaced by a pH indicator litmus solution to visualize the pH shift from neutral (violet) to acidic pH (red) over time due to citric acid pe*******on from the upper chamber to the bottom chamber, observed in the control group. In contrast, both the blank group and ST10 group exhibited no obvious colour change during the assessment, indicating their robust isolation effect of the modified transwell chamber membranes in these groups.
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-EtchedDentinSlice