06/04/2026
Many subcellular structures are defined not only by molecular identity but also by their spatial organization, morphology, and cellular context. As reviewed in a recent Molecular & Cellular Proteomics article, conventional proximity labeling methods typically rely on genetic fusion or antibody-mediated targeting of labeling enzymes, which can limit analysis to predefined molecular targets.
To address this challenge, the review highlights microscopy-guided subcellular proteomics (optoproteomics), which combines fluorescence imaging with targeted photolabeling of proteins within visually selected subcellular regions. By enabling proteomic profiling based on spatial features and morphology, this approach can characterize structures such as stress granules and amyloid-β plaques with high spatial specificity and sensitivity.
https://www.sciencedirect.com/science/article/pii/S1535947626000150
