Applied BioPhysics

12/10/2025

🥳 research from the University at Albany CNSE

Led by Fernando Pesantez Torres, this study used ECIS to track how NIH 3T3 fibroblasts and bEnd3 endothelial cells respond to SMA nanoparticles in real time.

🧪 Negligible cytotoxicity at all doses
🛡️ High NP levels slightly slowed barrier formation
🔄 Medium change restored barrier function at low doses; high doses remained reduced, likely from NP attachment

The results underscore ECIS as a powerful platform for monitoring cell behavior and guiding the safe design of oxygen-sensitive nanomaterials for biomedical use.

📖 Read the full study: https://pubs.acs.org/doi/10.1021/acsabm.5c01443

10/14/2025

Hyperosmolar stress cut corneal barrier integrity by up to 69%—but L-carnitine helped restore function. Using Z-Theta, researchers showed:

🔹NaCl stress reduced TER by 30–69% and slowed migration
🔹 L-carnitine partially rescued TER and improved repair.
🔹 ECIS enabled automated wounding and real-time recovery tracking, outperforming scratch assays.

✨ Findings point to L-carnitine’s therapeutic potential in dry eye disease and the value of in barrier research.

📄Full Paper: 10.1016/j.exer.2025.110499

10/02/2025

Using Z-Theta, researchers tracked how Müller glial cell EVs affect RPE barrier function:

🔵Normoxic EVs (blue): Barrier stays stable ✅
🟡Hypoxic EVs (yellow): Barrier weakens quickly, TER drops over 72h ⚠️

💡 Label-free, real-time monitoring shows how hypoxic EVs compromise retinal integrity—critical for diseases like diabetic retinopathy.

📄Full paper: https://www.mdpi.com/2079-7737/14/8/1014 #

08/27/2025

Insulin-regulated palmitoylation plays a crucial role in maintaining the integrity of lymphatic endothelial cell (LEC) barriers. Researchers at Saint Louis University used to continuously monitor TEER in real-time under incubation conditions, revealing how changes in palmitoylation profiles directly affect LEC permeability and stability.

🔗 Study: https://lnkd.in/gu9sdwqJ
ℹ️About ECIS: https://lnkd.in/gEqFH5Bt

07/10/2025

In Loving Memory of Dr. Ivar Giaever (1929–2025)

It is with heavy hearts that we share the news of the passing of Dr. Ivar Giaever. Ivar passed away peacefully on June 20, 2025, at the age of 96, surrounded by his family.

Ivar was not only a Nobel Laureate in Physics—recognized in 1973 for his pioneering work in superconductivity—but also a visionary who brought that same spirit of curiosity and innovation to the life sciences. His work led to the development of Electric Cell-substrate Impedance Sensing (ECIS), a groundbreaking method for studying living cells in real time using electrical currents—without labels or dyes.

In 1991, he co-founded Applied BioPhysics with the mission of sharing ECIS technology with the broader research community. Thanks to his leadership and vision, ECIS has become a trusted tool in labs around the world, helping to advance cancer research, drug development, toxicology, and countless other areas of science.

To those of us who had the privilege of working with him, Ivar was more than a brilliant scientist—he was a kind, witty, and inspiring presence. He challenged us to think differently, to question everything, and to approach science with both rigor and imagination. His mentorship, humor, and boundless energy shaped the culture of our company and left a lasting impact on all of us.
We will miss him deeply, but we carry forward his legacy every day—in our work, our values, and our commitment to supporting the scientists he cared so deeply about.

Our thoughts are with his family, friends, and all who were touched by his extraordinary life.

05/16/2025

🎉Applied BioPhysics, Inc. turns 34 today! 🎈 Thanks to everyone who's been part of the ride. Let’s keep pushing science forward!🚀

Send a message to learn more

05/13/2025

🔎Did You Know? The first paper was published in 1984—the same year The Terminator warned us about Skynet and the Macintosh redefined computing.🍏🤖💻

Here’s to 40 more years of transforming science and pushing the boundaries of possibility! 🥳

https://www.researchgate.net/publication/16609706_Monitoring_fibroblast_behavior_with_an_applied_electric_field

04/22/2025

♻️ From thoughtful operations to waste reduction, we’re committed to doing science the right way—for people and the planet🌎.

04/18/2025

🧪 How do you track lung cell injury in real-time—without disturbing your cells? Use 😉

Researchers at Shanghai Jiao Tong University tackled sepsis-induced lung injury🫁 by exploring the protective power of telocyte-derived exosomes🧬 —and uncovered a key role for miRNA-146a-5p in blocking cell death via the caspase-3 pathway.

But here’s the cool part 👉 They used hashtag to monitor endothelial barrier changes live—while the cells stayed incubated. No disruptions. Just precise, continuous data 📊🔥

💡 Want to see what hashtag can do for your endothelial cell research?

🔗Contact Us: https://lnkd.in/gEqFH5Bt
📚 Study link: https://lnkd.in/gJu5-JBX

04/11/2025

🔬 Using ECIS, researchers tracked real-time effects of 🐍viper and cobra venoms on human lung cells🫁—and showed that DTIs like varespladib and marimastat can effectively block this cytotoxicity.

🎉A recent study from Abhinandan Chowdhury, Ph.D. and the Adaptive Biotoxicology Lab (UQ) highlights the power of in venom research.

🐍 ECIS detected significant cell detachment and damage
🛡️ DTIs offered broad, cross-species protection
⚖️ Coagulation assays confirmed DTIs neutralize venom effects

We're proud to see enabling this important work and supporting alternatives to animal testing in toxicology. Check out the full paper below 👇

📄 https://www.mdpi.com/2072-6651/17/4/193

04/04/2025

New Tech Alert! 🚀 The TEERZ station is here to improve your ECIS experience with continuous, real-time TEER readings—say goodbye to those manual endpoint measurements! 🙋‍♀️ It pairs perfectly with standard filter inserts and your ECIS Z-Theta for even more data without any extra work. 🔥 Take your research to the next level with TEERZ! 💡